specimens: Place swab in Universal Transport Medium (UTM-RT) and transport at room temperature for up to 24 hours after collection or refrigerate.

defined media prepared from pure chemical substances. 122 0 obj <> endobj �/\���� h�bbd``b`n�׀C�`�$�/ � �� 1s ��$$� �r��'�P��5�i � �o#l����;&F.������4� @� ��| Pike’s medium is used to transport streptococci from throat specimens. x��X]S�6}ϯ�c2�]Y��}�t��$�K�qqlj���/���/��R��'�9�H�Cz�E��$� ��I(���H�t�;�����~��h������4�B� 80|0�������b�����qD ��;��N.�|]��So�{��'���}bL~�OP��� T���;��W��r���Q�-�4��u�h��4ņ�u�z�#��΁�*���qP�@#�g�M��13�yސ��s���Yxy�WH�*w,T����;7z�%�r�M5���"TTb!z�}0K�M��Q{J%�-� ����%#���b/F�p�(9�O���(���������0��=� ���&ϗ���B��ջe�A���φ����JY#@���1�? endstream endobj startxref

%PDF-1.6 %���� �,Mj�t���j�r�Z#b� endstream endobj 25 0 obj <>stream Urine, csf and other body fluids do not have to be in viral media. A variety of transport media have been … %%EOF Solid medium: – Solid medium contains agar at a concentration of 1.5-2.0% or some other, mostly inert solidifying agent. SUMMARY AND EXPLANATION Transport media are designed to preserve the viability of microorganisms when immediate inoculation of growth media is not possible. It is used in research work.

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should be preserved in viral transport media available in the Microbiology laboratory and transported on ice. Microbiology Collection Media and Specimen Transport Specimen Type (by source or test) Preferred Collection Media & Media Storage Storage after Collection Comments BD ESwab: Nylon flocked swab (regular, minitip, flexible minitip) with 1 mL modified liquid Amies Room temperature Sustains viability of aerobic, anaerobic, and 143 0 obj <>/Encrypt 123 0 R/Filter/FlateDecode/ID[<52CB34E32F711B4C91BF28B39A11970D>]/Index[122 52]/Info 121 0 R/Length 97/Prev 545353/Root 124 0 R/Size 174/Type/XRef/W[1 2 1]>>stream

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Microbiology Collection Media Guideline MIC-101-I Version: 01 Effective Date: March 21st, 2016 Page 1 of 3 Body Fluid Culture Sterile Collection Container, Syringe (Needle removed) N/A Bordetella Pertussis Culture/PCR BD Amies Charcoal Swab Genital Culture BD Red top Liquid Stuart Copan swab Group B Streptococcus BD E-Swab @��l�x��,)�r�鬽 U�*{)a�� ��*,@���#�>����:GQe�� �pn�L�O��9}ڽ��V&a��)����l5�$�*#��ǝ"^H[Kkd��bJ�O�_)���t��*t;�� ��C=�%w�64�"#�@�>ĩ���������27̍�ӄ��'���Þ�0�6����6�LA*V0�k-|���a�e��]�+����=)S���H۴�:�e^Iρ�����Y�!��#�R1[ֱ|%`'��v;ۡ�]9&*�8�#�p�nW��t[iX2��= oߝ��^����A�ƢWBRT�"���f-o�I�r,�������Ž�?H��]�e`���z�1�ѯ�w+�`��6���ӗuw����� �و&���(����q��DM�����x��cz�� Transport media are essentially solutions of buffers with carbohydrates, peptones and other nutrients (excluding growth factors) designed to preserve the viability of bacteria during transport without allowing their multiplication.The primary objective of the use of the transport medium is to maintain the specimen as near its original state as possible. 21 0 obj <> endobj

�21�ZV�du�@v��~��h` ���=�����4�?t]�����Ȃ��C�����R�tN��{^}��?�������5)�Zٱ�^��s��RB[^�y[��&X��o���֍�^�����Į;������2`E���9zL���J�~��Zq�/2�~׬�2�×��N)4-�S�H+G�1���{�� UU���y�Ӿ��E�Ѱ��Lq#���1��@v�Z#���4n�>�C�ô�*��7����~2i�Xc�JD��N�p'���9% jca���-�M��Z��; Ȭ4�-w4�n� E�'����FI>#'�i$�2 Transport specimens immediately at ambient temperature. Cary Blair transport medium and Venkatraman Ramakrishnan (VR) medium are used to transport feces from suspected cholera patients.

Amies Transport Medium. Transport Medium was originally described by Stuart, in 1946.1 Moffett et al. {�L����R���c!4��x�[ @RV������j�6� G�wr� H>CH�K�h��'��Ș��$B��t�/KH�A"=�!�N�(}���O9�nQ/�DZdE;g��X���֌��?���C����@;�#E��'����t,^?�k�-��BG�Ei��z��y6�A���m���2= ��������z�r�� O�:+��$i��f��Z‚��J>��y�r. �v�~�QF�n��Z�h�4>N��W�r�ݮ!�5Qb��� �մ��>qu�qc�pQx�5n�u#*C��c�&_A��\k>3���� '�rwD�f�"�}���"��M��IEe���6�A��4���'�X���tbi�^���(� Obtain collection system from Microbiology lab, Meyer B-171, 955-6510 option #1. MICROBIOLOGY MODULE Bacterial Culture Media Microbiology 102 Notes zclassify the culture media zdescribe the preparation and storage of Culture media When culturing bacteria, it is very important to provide similar environmental and nutritional conditions that exist in its natural habitat. Supply order number: 93307 Bordetella Culture Transport Charcoal-containing Regan-Lowe medium is used for transport of specimens for B pertussis culture. It is a semisolid media recommended for use in qualitative procedures for the transport of …

114 0 obj <>stream All specimens for virus culture may be refrigerated at 2-8C for 24 hours. endstream endobj 8 0 obj <>>>/Filter/Standard/Length 128/O(�mޢ܁!�$x &�:�������GQG)/P -3100/R 4/StmF/StdCF/StrF/StdCF/U(��m��IN@L���Mm )/V 4>> endobj 9 0 obj <>>> endobj 10 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/Properties<>/XObject<>>>/Rotate 0/TrimBox[0.0 0.0 972.0 1404.0]/Type/Page>> endobj 11 0 obj <>stream ޟe!�"�f�D!�4b�F������ނ%-[��Qb���/�9��X���Ke��1u ɋ��(3�z��0攛Qz(��id�uO��o4/qN�B J[���)�J=�uTA�5����l��

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Transport and Culture Medium Jembec Plate for GC Culture Culture media transport system containing Modified Thayer Martin agar used to improve recovery of N gonorrhoeae. [�Km��Aʟt��Dg�e��:Niؓ Collection of specimens with swabs that are toxic to either virus or cell culture should be avoided. %PDF-1.6 %���� /,l��5��98߶U�W��Ӓ0��k\vN]Gi��-Sj!�����p�H` ��e��]�W�;8�M+x�u���{l5�fJ���t�u�=o$��L���d4��x�N�{��1�k�xE��V��������t�q�,In� �kC]�I�S��x�vXE�k� ������՚�^���̀�H���)�yN�����F�ǘ'��]�TI��� These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective media, (4) Indicator media, (5) Transport media, and (6) Storage media. 40 0 obj <>stream �}��0 �R�N����B�'��p�g�0E13:�'�Y��7�ڢ,�tf�I���x�p� �b*�P5���Ğ�pH��%�efxKQ�R4�;v�,�yBs�{��@���T⒮�����r��c ��̪��ѕ-˲��t����m.ѣ� D�P� &R� 3�T[L��,��G��I� V�R��d�M}���� G ,�-�w�.�2�fC[���*޲��h+��?���+9�o^���:]S gG��\f9(}b�CP��r�Q�K�����CI���2 \g�˽�ҕ�S~,4G��tM�$�����J�;� f: ���j���Aq��Hn��&N�����,���5�3���B2p���-�X\���%���rc���T�{/3��:WV(Bb}xz�g����+��>E%z�$k�2�޼�}��'�����:(�"�q���av��3�'P�3�۰0�����<0#&�FͶf��#?���cHE �֟. $�zբabv@uv�/= �}��9vFyD�lp��u`�U�����L�p(���Dϻ�NH&IY���RGTq��q�gص]Ao�͏�M%���i�o�FPr����'���A��� ��楴�����\�` >�T�e�2��d���^ʎE��Y�����ǎ�4_#���aa�7#�z Bۻ�N[m�>�{=P�l �>]��l��=�3H0���� endstream endobj 123 0 obj <>>>/Filter/Standard/Length 128/O(�mޢ܁!�$x &�:�������GQG)/P -3100/R 4/StmF/StdCF/StrF/StdCF/U(#�ؽ9h�� I@uY�7� )/V 4>> endobj 124 0 obj <>>> endobj 125 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/Properties<>/XObject<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 126 0 obj <>stream

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Stuart et al (7) later on modified the Stuart Medium for the transportation of gonococcal specimens for culturing. 29 0 obj <>/Filter/FlateDecode/ID[<739FA930CC21DE9FA1AAF1B18F38737A>]/Index[21 20]/Info 20 0 R/Length 64/Prev 261370/Root 22 0 R/Size 41/Type/XRef/W[1 3 1]>>stream h�bbd```b``��� �� ����� R�H2��������V��00�L�g`�� � �� endstream endobj startxref 0 Microbiology Sample Collection and Transport Collection of specimens for culture differs in two ways from collection of specimens for routine analysis: 1. 0 BASAL MEDIA. K��Hu�[�Ϟq���`�H�F�z��kBe�և�r��f����^D�a4�T}�Z�����%D��"��?

applied the Stuart formulation to preserve viability of fastidious organisms, including Neisseria spp., during transport from clinic to laboratory.2 In 1954, Stuart et al. %PDF-1.7 %����

Ringertz included thioglycollate in the Stuart Medium and omitted charcoal (5). 88 0 obj <>/Encrypt 8 0 R/Filter/FlateDecode/ID[<9F5090074E762F43B8FE91D17C398318><8542C34D7320A745A1E75A46FD7B15BA>]/Index[7 108]/Info 6 0 R/Length 293/Prev 460957/Root 9 0 R/Size 115/Type/XRef/W[1 3 1]>>stream 6. 0 ��_OE�h�����uM�Kx(�J�� >������0qO�M}]�0� \���܄4��\b endstream endobj 22 0 obj <> endobj 23 0 obj <> endobj 24 0 obj <>stream Provided in the collection system are two swab/swab transport packages. 1. %%EOF