Veins on the underside of the wrist should be avoided.
If herpes, Chlamydia, or pre-cancer is considered, then the clinician should IMMEDIATELY place slide in 95 percent ethanol and submit for Pap stain. GUIDELINES FOR FINE-NEEDLE ASPIRATION (FNA).
Obtaining reliable and accurate laboratory test results If slides are made, print the patient’s last name and a second identifier with pencil on one end of all frosted slides. Residents are available for questions but do not assist with FNA slide preparation and assessment of adequacy. Together we care for our patients and our communities. Specimen Collection. These are rich in exfoliated cells from the bronchial epithelium and are of great diagnostic value. Hours: Monday - Friday: 8 a.m. - 5 p.m. EST The drop is permitted to spread across the joining edges of the slide, and at that point, the forwarding slide is pushed across the surface, smearing the sample. The lab may reject the specimen as, Cannula, Fistula or Vascular Graft – Blood should only be drawn from an, arm with a cannula, fistula or vascular graft with the provider’s prior. regardless of how small it may be. The delivery to the Cytopathology Lab is delayed, the fluid samples must be refrigerated. During this procedure, the nurse should. If drawing above the IV site is the only option, then the IV infusion, must be turned off for at least 2 minutes before performing the, venipuncture. If the specimen cannot be brought immediately to the lab, it MUST be refrigerated. arm vein. Select the. Blood Collection … Several essential steps are … Specimen Collection Procedures and Policies Blood / Body Fluid Precautions All specimens which contain blood or body fluids must be considered infectious. Blood Specimen Collection and Processing. If it cannot be immediately delivered, add three units of heparin per mL of fluid to prevent clotting, and place in refrigerator until it can be delivered. A nurse caring for a group of patients in an ambulatory care clinic is collecting urine for several prescribed diagnostic tests. Grasp the patient’s arm firmly using your thumb to draw the skin taut and, anchor the vein; do not use the index finger to pull the skin upward as this, increases the risk of sticking yourself.
A Supervisor or Manager should be notified and the incident. A nurse is teaching a patient about home collection of a stool specimen for fecal occult blood testing. h�bbd```b``���� ��̖��A$�/��|"�+��^`RLf�� Intravenous therapy/Blood Transfusions – If it is not possible to draw the. Which of the following actions should the nurse take when obtaining a wound culture specimen from the pressure ulcer? Continue to collect till morning. Patience with scheduling is appreciated. UF Health Pathology Laboratories Nontuberculosis Mycobacteria Laboratory, Specimen Collection Procedures: Hematopathology, Specimen Collection Procedures: Microbiology, Surgical Pathology Shipping Instructions: Muscle Biopsies, Surgical Pathology Shipping Instructions: Nerve Biopsies, Surgical Pathology Shipping Instructions: Renal Biopsy, UF Health Shands Children's 1. Materials provided by the laboratory for specimen collection can maintain the quality of the specimen only when they are used in strict accordance with the instructions provided. During this procedure, the nurse should A. keep the tourniquet in place from selection of the vein to completion of the collection B. rub the patient's arm at the selected site prior to venipuncture Northfield, IL: College of American, NCCLS. After cleansing the area, if it is accidentally. Method of collecting 24 hours urine specimen: 24 hours urine specimen means to collect all the urine voided in 24 hours. The first step in acquiring a quality lab test result for any patient is the specimen collection procedure. Cerebrospinal fluid (CSF): Bring the fresh specimen PROMPTLY to the Lab for processing. 2) All specimens must be capped/sealed tightly. The site must have all necessary personnel, supplies, equipment, facilities, and supervision to provide for specimen collection, security, and temporary storage until the specimen is Apply a band-aid or CoFlex to, the site. As there is still a risk that the sample could be, contaminated, you must document that the specimen was drawn above, (proximal to) an IV site and how many minutes the IV was turned off, before the draw occurred. This article, the first in a four-part series, explores the general principles of specimen collection and describes how to collect a midstream specimen of urine (MSU). The Cytology Laboratory staff assists in fine-needle aspiration (FNA) procedures in various areas at UF Health Shands Hospital and only during regular hours (8:30 a.m. – 3:30 p.m.). The slide, which must have the patient’s last name and 2nd identifier written on the frosted end, is given an accession number and submitted to Histology for a Gomori methanamine silver stain and evaluated for the presence of fungi or Acanthamoeba. The upper extremity on the side that a mastectomy was performed. Palpate and trace the path of the vein with the index finger.
We prefer at least 30 mL of sample or more if possible. Air-dried smears are helpful for special stains and immediate evaluation of adequacy using rapid stains. Together we teach. When immediate assistance is not available: It is recommended for aspirate be placed into CellGro (RPMI or DMEM). Smear the brushing or aspirated material and fix the slide immediately in 95 percent ethanol.
�=�Ʋ|�*@3�:&!�X��V�:�ó���W�S���ޓ,��v�=����sy<�Y�|SQ�#�4J�sTW���)������۲�A�����Lpttt00�t q�! We prefer a fresh unfixed samples. If there is not an alternate vein to draw. “Pediatric Pointers.” Center for Phlebotomy Education, Inc. Ernst, Dennis J. and Catherine Ernst. IMMEDIATELY to the Cytopathology Lab after collection.
The procedure used to collect a specimen must minimise the risk of introducing error and protect the health and safety of both the patient and the staff who handle the sample. As much of the aspirate as possible should be placed in CellGro (DMEM or RPMI) for possible flow cytometry in suspected cases of lymphoma. Gastrointestinal tract: Brushing or aspirated material may be smeared directly on slides, which should have the patient’s last name and a second identifier printed in pencil on one end. Make sure the, needle fully penetrates the upper-most wall of the vein; partial penetration, may allow blood to leak into the tissue surrounding the vein. An assistive personnel (AP) is collect a 24-hour urine specimen from a patient. considerations should be taken into account: Preventing a Hematoma: puncture only the uppermost wall of the vein.
In the case of a difficult venipuncture, an, individual may make a maximum of two attempts before having, someone else try. Which of the following instructions should the nurse provide. Do not dry the site with gauze and do not blow on the area to dry as this, will contaminate the site. h�b```b``������-� Ȁ �@1V ����(���������!��y���7T�q�sT7�Xΐ�`�Al����,�&2�7l��P���2�qu��s�g(7���.gj�ը����C�¬�wｽ��.��w�r���\h�G(vQc_��yN}�����LovI^x>��{K���U\�xQ|c¤=%�o|���1����vr���@�qpD�mF�� �F)e���BBq��}@��:�U^ ��X�n�H� Specimen collection and processing procedures Proper specimen collection and handling is an integral part of obtaining a valid and timely laboratory test result. After the drop spreads from the weight of the slide, rotate the slides apart by turning the upper-slide away from the lower-slide, like turning the page of a book.
endstream endobj 2090 0 obj <. 8H C(1du`#T�"�$5*À������ �4�\ˈb�2D 5� �Ж4��f@��%��.����'6�G�[�t���+g��/Ppr`�7dܧ!x��÷KR�n�� �ml%�@Z �a�`��_ '�{�XAC��p�[`UZ+ch��'�7��d��>6�_�C�C�uq�����Ü��w6�4�0 ]�����g�7x ���(�=�^��.�uȟ%l�G��C� �8� ����`5Dc@� 1�T� Phone: 352.265.9900 additional specimens. retract from the vein and the safety device will cover the needle. Specimen Collection • Suspect or confirmed TB patients should be in a negative pressure room • Specimen collection is an aerosol generating procedure, anyone in the room during specimen collection must wear a particulate respirator type N-95 and be part of the respirator protection plan • All mycobacteria specimens are collected The health technician logs onto the specimen collection application and logs the SP into the component. Scrape the wound with a moistened tongue blade and place the material directly on the slides. In this technique, a second slide is simply brought up to the drop.
Collection Procedure: LTD-Stool: Specimen Collection Version 6. Venipuncture; Approved Standard—Sixth Edition. Collection guidelines (other than FNA) 1) Contact laboratory before obtaining cerebrospinal fluid specimen on patient suspected to have Creutzfeldt-Jakob disease. For cytomegalic inclusion disease (CMV) or polyoma (BK virus) detection: Fresh urine should be sent Butterfly needles. arm to start bleeding when the arm is straightened out. Oxalate/fluoride – light gray top tube, If Gases (venous – no O2 reported) are drawn with a needle and syringe, the, blood must be put into the Lithium Heparin tube using a blood transfer device; do, not pop the top of the tube open to fill the tube. Bronchial: Send fresh bronchial secretions or washings to the Cytopathology Lab immediately, or refrigerate specimens until they can be delivered to the Lab for processing. Pneumatic Tube System Procedure.
See below. Smears: Smears must be labeled with the patient’s last name and a second identifier. A nurse is caring for a patient who has a stage 3 pressure ulcer in the sacral area. Edematous extremities – tissue fluid accumulation can alter test results. Gainesville, FL 32608, Hours: Monday - Friday: 8 a.m. - 5 p.m. EST. minutes and then reapplied before performing the venipuncture.
Accept no more than two specimens/patient without prior consultation. with an individual who can explain the limited yield provided by. Vacutainer tube holder 5. The nurse is collecting a blood specimen for culture from a patient hospitalized for pneumonia. 21g or less 3. SST red or gold top – this tube contains a gel separator and clot, 9. DO NOT ALLOW THE SMEAR TO AIR-DRY. 3.Fine needle aspiration may also be used for breast masses. Phelbotomy Policies and Procedures. The minimum CytoLyt® to sample ratio should be 1:3.
Adapted from Phlebotomy for Nurses and. IMMEDIATELY place the slides in 95 percent ethanol, or spray them with an appropriate fixative, keeping the spray nozzle 12 inches away from the slide surface. Re-attach the needle and advance the plunger of the syringe to express a small drop of the aspirated material on the center of the glass side. A nurse is caring a patient who has a suspected urinary tract infection (UTI). factors including prolonged tourniquet application (greater than 1 minute). Should an FNA be anticipated after 3:30 p.m., contact the FNA service during operating hours for needed supplies. The venipuncture should be repeated in a, different site. venipuncture site by palpating with the gloved index finger. over the venipuncture site.
2. All containers must be tightly capped or sealed to prevent leakage/spillage and placed in a plastic bag that has been approved for … 1) Contact laboratory before obtaining cerebrospinal fluid specimen on patient suspected to have Creutzfeldt-Jakob disease. Adequate, pressure should be applied to stop the bleeding once the phlebotomy is, complete. Arteries pulsate, are, more elastic and have a thicker wall than a vein. Which of the following urinalysis results should indicate to the nurse the presence of a UTI? a bed. 2) All specimens must be capped/sealed tightly. Related Pages. They are prepared from a small drop of the semisolid aspirate placed on a glass slide. The rest of the material can then be placed in the transport fluid.